Premium
Diminished presentation of complement regulatory protein CD 55 on red blood cells from patients with hereditary haemolytic anaemias
Author(s) -
LoniewskaLwowska A.,
Koza K.,
MendekCzajkowska E.,
Wieszczy P.,
AdamowiczSalach A.,
Branicka K.,
Witos I.,
SapalaSmoczynska A.,
Jackowska T.,
FabijanskaMitek J.
Publication year - 2018
Publication title -
international journal of laboratory hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.705
H-Index - 55
eISSN - 1751-553X
pISSN - 1751-5521
DOI - 10.1111/ijlh.12752
Subject(s) - microcytosis , hereditary spherocytosis , red blood cell , immunology , flow cytometry , antibody , spherocytosis , chemistry , microbiology and biotechnology , red cell , monoclonal antibody , medicine , andrology , iron deficiency , biology , anemia , splenectomy , spleen
Hereditary haemolytic anaemias ( HHA ) encompass a heterogeneous group of anaemias characterized by decreased red blood cell survival. The aim of this study was to evaluate the status of red blood cell ( RBC ) surface molecules known or previously proposed to participate in preventing premature RBC clearance, analysing erythrocytes from patients with two types of HHA : hereditary spherocytosis ( HS ) and microcytosis. Material/Methods Relative binding of five monoclonal antibodies ( mA bs), anti‐ CD 55, anti‐ CD 59, anti‐ CD 44, anti‐ CD 47 and anti‐ CD 58, was evaluated in erythrocytes of patients with HS and hereditary microcytosis, using flow cytometry. The amount of CD 55 protein was assessed by semi‐quantitative Western blots densitometry analysis. Results The majority of both HS and microcytic patients demonstrated significant reduction of anti‐ CD 55 binding by erythrocytes (average 23% and 19%, respectively, P < .001), with no concomitant anti‐ CD 59‐binding deficiency. Anti‐ CD 44, anti‐ CD 47 and anti‐ CD 58 binding was within the healthy control range or was slightly decreased. Conclusions This study provides evidence supporting the presence of erythrocytes deficient in CD 55 presentation in HS and hereditary microcytosis. Moreover, deficiency of CD 55 antigen presentation on RBC does not correlate with the amount of CD 55 in RBC membrane. Further studies using molecular techniques will clarify the exact participation of CD 55 deficiency in premature RBC clearance in HHA .