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Comparison of quantitative and qualitative tests for glucose‐6‐phosphate dehydrogenase deficiency in the neonatal period
Author(s) -
Keihanian F.,
Basirjafari S.,
Darbandi B.,
Saeidinia A.,
Jafroodi M.,
Sharafi R.,
Shakiba M.
Publication year - 2017
Publication title -
international journal of laboratory hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.705
H-Index - 55
eISSN - 1751-553X
pISSN - 1751-5521
DOI - 10.1111/ijlh.12618
Subject(s) - medicine , cord blood , predictive value , glucose 6 phosphate dehydrogenase deficiency , gastroenterology , pediatrics , physiology
Summary Introduction Considering the high prevalence of glucose‐6‐phosphate dehydrogenase (G6 PD ) deficiency among newborns, different screening methods have been established in various countries. In this study, we aimed to assess the prevalence of G6 PD deficiency among newborns in Rasht, Iran, and compare G6 PD activity in cord blood samples, using quantitative and qualitative tests. Methods This cross‐sectional, prospective study was performed at five largest hospitals in Rasht, Guilan Province, Iran. The screening tests were performed for all the newborns, referred to these hospitals. Specimens were characterized in terms of G6 PD activity under ultraviolet light, using the kinetic method and the qualitative fluorescent spot test ( FST ). We also determined the sensitivity, specificity, negative predictive value, and positive predictive value of the qualitative assay. Results Blood samples were collected from 1474 newborns. Overall, 757 (51.4%) subjects were male. As the findings revealed, 1376 (93.4%) newborns showed normal G6 PD activity, while 98 (6.6%) had G6 PD deficiency. There was a significant difference in the mean G6 PD level between males and females ( P = 0.0001). Also, a significant relationship was detected between FST results and the mean values obtained in the quantitative test ( P < 0.0001). Conclusion According to the present study, FST showed acceptable sensitivity and specificity for G6 PD activity, although it appeared inefficient for diagnostic purposes in some cases.