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Evaluation of two automated cell counters for the analysis of hematopoietic progenitor cell apheresis products
Author(s) -
Gils S.,
Cauwelier B.,
Devos H.,
Vanlaere I.,
Roggeman S.,
Emmerechts J.
Publication year - 2017
Publication title -
international journal of laboratory hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.705
H-Index - 55
eISSN - 1751-553X
pISSN - 1751-5521
DOI - 10.1111/ijlh.12617
Subject(s) - apheresis , hematocrit , blood cell , hematology , white blood cell , haematopoiesis , medicine , progenitor cell , hematology analyzer , platelet , red blood cell , immunology , stem cell , biology , genetics
Summary Introduction Routine hematology parameters in hematopoietic progenitor cell apheresis products ( HPC ‐A) are usually determined using automated cell counters. These instruments, however, are designed to analyze whole blood samples, that differ considerably from HPC ‐A in blood cell composition. This study evaluates the performance of two automated cell counters for the analysis of HPC ‐A. Methods Routine hematology parameters [red blood cells ( RBC ), hematocrit ( HCT ), mean corpuscular volume ( MCV ), white blood cells ( WBC ), WBC differentiation, and platelets ( PLT )] were determined on the Unicel DxH 800 instrument (Beckman Coulter) and the XN ‐350 instrument (Sysmex). Correlations with the reference methods, intrarun precision, and linearity of the analyses were studied. Results Good correlations were found for almost all parameters. However, RBC count was overestimated by XN ‐350, using the impedance technique, as was neutrophil percentage using DxH 800. Coefficients of variation for intrarun precision were below 10% on both analyzers for all parameters, except for neutrophil percentage (14.7%) and PLT (10%) on DxH 800. Both instruments showed good linearity for all parameters, except for RBC and HCT on DxH 800. Conclusion With the exception of the measurement of neutrophils on DxH 800 and RBC by the impedance technique on the XN ‐350, routine hematology parameters in HPC ‐A can safely be determined using automated cell counters.