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Proliferative characteristics of Philadelphia‐negative myeloproliferative neoplasms ‐ clinical implications
Author(s) -
Šefer D.,
BižićRadulović S.,
KraguljacKurtović N.,
Bogdanović A.,
Čokić V.,
Miljić P.,
BeleslinČokić B.,
Knežević V.,
MitrovićAjtić O.,
Leković D.,
Gotić M.
Publication year - 2017
Publication title -
international journal of laboratory hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.705
H-Index - 55
eISSN - 1751-553X
pISSN - 1751-5521
DOI - 10.1111/ijlh.12564
Subject(s) - myelofibrosis , essential thrombocythemia , polycythemia vera , cd34 , flow cytometry , bone marrow , cfu gm , immunology , andrology , microbiology and biotechnology , medicine , biology , pathology , haematopoiesis , stem cell , genetics
Summary Introduction Philadelphia‐negative myeloproliferative neoplasms (Ph − MPN ) are characterized by overproduction of one or more blood cell lines. Methods We studied the proliferative characteristics of 91 patients with de novo Ph − MPN . Colony‐forming cells ( CFC ) and endogenous colonies ( EC ), from bone marrow ( BM ) and/or peripheral blood ( PB ), were analyzed by colony assay based on methylcellulose. The level of circulating CD 34 + cells was determined by flow cytometry. Results The total number of PB CFC in primary myelofibrosis ( PMF ) was increased compared to the control sample ( P < 0.01) and essential thrombocythemia ( ET ) ( P < 0.05). The highest number of BM and PB EC was observed in polycythemia vera ( PV ) ( P < 0.01). Increased levels of CD 34 + cells characterized early‐prefibrotic (57%) and advanced‐fibrotic PMF (90%) as compared to PV (34%) and ET (32%) ( P < 0.01). In the whole Ph − MPN group, the total number of PB CFC ( P < 0.01), PB EC ( P < 0.05), and CD 34 + cells ( P < 0.01) correlated with the degree of BM fibrosis. Higher levels of circulating CD 34 + cells in PMF correlated with the total number of PB EC ( P < 0.05) and degree of BM fibrosis ( P < 0.01). Conclusions Exploration of the PB proliferative characteristics of Ph − MPN on diagnosis may be helpful in revealing early‐prefibrotic PMF . Monitoring the levels of circulating CD 34 + cells may provide a sensitive indicator of fibrotic evolution in PV and PMF .