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Evaluation of three fully automated immunoassay systems for detection of IgA anti‐beta 2‐glycoprotein I antibodies
Author(s) -
Pérez D.,
MartínezFlores J. A.,
Serrano M.,
Lora D.,
PazArtal E.,
Morales J. M.,
Serrano A.
Publication year - 2016
Publication title -
international journal of laboratory hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.705
H-Index - 55
eISSN - 1751-553X
pISSN - 1751-5521
DOI - 10.1111/ijlh.12543
Subject(s) - kappa , antibody , immunoassay , glycoprotein , immunoglobulin a , secretory iga , medicine , immunology , immunoglobulin g , biology , mathematics , microbiology and biotechnology , geometry
Summary Introduction In recent years, we have been witnessing increased clinical interest in the determination of IgA anti‐beta 2‐glycoprotein I ( aB 2 GPI ) antibodies as well as increased demand for this test. Some ELISA ‐based diagnostic systems for IgA aB 2 GPI antibodies detection are suboptimal to detect it. The aim of our study was to determine whether the diagnostic yield of modern detection systems based on automatic platforms to measure IgA aB 2 GPI is equivalent to that of the well‐optimized ELISA ‐based assays. Methods In total, 130 patients were analyzed for IgA aB 2 GPI by three fully automated immunoassays using an ELISA ‐based assay as reference. The three systems were also analyzed for IgG aB 2 GPI with 58 patients. Results System 1 was able to detect IgA aB 2 GPI with good sensitivity and kappa index (99% and 0.72, respectively). The other two systems had also poor sensitivity (20% and 15%) and kappa index (0.10 and 0.07), respectively. On the other hand, kappa index for IgG aB 2 GPI was >0.89 in the three systems. Conclusion Some analytical methods to detect IgA aB 2 GPI are suboptimal as well as some ELISA ‐based diagnostic systems. It is important that the scientific community work to standardize analytical methods to determine IgA aB 2 GPI antibodies.