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Efficacy of DSP 30‐ IL 2/ TPA for detection of cytogenetic abnormalities in chronic lymphocytic leukaemia/small lymphocytic lymphoma
Author(s) -
Holmes P. J.,
Peiper S. C.,
Uppal G. K.,
Gong J. Z.,
Wang Z.X.,
Bajaj R.
Publication year - 2016
Publication title -
international journal of laboratory hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.705
H-Index - 55
eISSN - 1751-553X
pISSN - 1751-5521
DOI - 10.1111/ijlh.12513
Subject(s) - immunology , cytogenetics , lymphoma , biology , medicine , cancer research , microbiology and biotechnology , gene , genetics , chromosome
Summary Introduction Chronic lymphocytic leukaemia (CLL) is the most prevalent leukaemia in the Western Hemisphere. Cytogenetic abnormalities in CLL are used for diagnosis, prognosis and treatment. However, detecting these is difficult because mature B cells do not readily divide in culture. Here, we present data on two mitogen cocktails: CpG‐oligonucleotide DSP 30/Interleukin‐2 ( IL ‐2) and DSP 30/ IL ‐2 in combination with 12‐ O ‐tetradecanoylphorbol‐13‐acetate ( TPA ). Methods We analysed 165 cases of CLL with FISH and cytogenetics from January 2011 to June 2013. In 2011, three cultures were set‐up: unstimulated, DSP 30/ IL ‐2‐stimulated and TPA ‐stimulated. In 2012–2013, two cultures were set‐up: unstimulated and stimulated with TPA / DSP 30/ IL ‐2. Results In 2011, FISH had a detection rate of 91% and cytogenetics using DSP 30/ IL 2 had a detection rate of 91% ( n = 22). In 2012–2013, FISH had a detection rate of 79% and cytogenetics using TPA / DSP 30/ IL ‐2 had a detection rate of 98% ( n = 40). The percentage of cases with normal FISH but abnormal cytogenetics increased from 9% in 2011 to 21% in 2012–2013. The TPA / DSP 30/ IL ‐2 cultures in 2012–2013 detected more novel abnormalities ( n = 5) as compared to DSP 30/ IL ‐2 alone ( n = 3). Conclusions TPA / DSP 30/ IL 2 was as good as or better than DSP 30/ IL 2 alone. TPA / DSP 30/ IL ‐2 offers a high detection rate for CLL abnormalities with a single stimulated culture and may increase detection of clinically significant abnormalities.