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Verifying the performance characteristics of the TEG 5000 thromboelastogram in the clinical laboratory
Author(s) -
Gosselin R.C.,
Estacio E. E.,
Song J. Y.,
Dwyre D. M.
Publication year - 2016
Publication title -
international journal of laboratory hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.705
H-Index - 55
eISSN - 1751-553X
pISSN - 1751-5521
DOI - 10.1111/ijlh.12464
Subject(s) - platelet , fibrinogen , whole blood , coefficient of variation , chemistry , medicine , chromatography
Summary Objective To verify the manufacturer performance claims of the TEG 5000 with traditional laboratory methods. Materials and methods Samples were concurrently measured using the TEG 5000 analyzer and either PT , APTT , fibrinogen, factor activities, platelet count, or platelet function testing using whole blood or platelet‐rich plasma methods. Results Within‐run imprecision yielded coefficient of variation ( CV ) of <5%. There was no correlation of PT or APTT with R time. Only Factor VIII and factor XII activity significantly correlated with R time. There was significant correlation between k and angle with FBG , PLT count, and factor levels. There was weak inverse correlation between angle results and measures of platelet function. All laboratory methods were significantly correlated with MA . There were significant differences between citrated whole blood and fresh citrated plasma for angle and MA , and between fresh and frozen plasma for R time and MA . We demonstrated a high % inhibition noted with normal, drug naïve donors, especially with ADP PLT mapping (50% inhibition), but less so with AA PLT mapping (20% inhibition). For TEG platelet mapping, 19/22 (86.3%) and 17/22 (77.3%) results were concordant with traditional aggregation results. Conclusion We demonstrated both the lack of, and strong correlation between laboratory tests and the TEG parameters.