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Expression analysis of BECN 1 in acute myeloid leukemia: association with distinct cytogenetic and molecular abnormalities
Author(s) -
ZareAbdollahi D.,
Safari S.,
Movafagh A.,
Ghadiani M.,
Tabarraee M.,
RiaziIsfahani S.,
Gorji S.,
Keyvan L.,
Gachkar L.
Publication year - 2016
Publication title -
international journal of laboratory hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.705
H-Index - 55
eISSN - 1751-553X
pISSN - 1751-5521
DOI - 10.1111/ijlh.12454
Subject(s) - becn1 , myeloid leukemia , autophagy , myeloid , cancer research , leukemia , npm1 , biology , immunology , gene , karyotype , genetics , chromosome , apoptosis
Summary Introduction In acute myeloid leukemia ( AML ), it has been shown that AML ‐derived cells often remain sensitive to autophagy‐inducing stimuli, leading to the idea that harnessing the autophagy can be pertinent to AML cytotoxic therapy. Despite this promising notion, to date, there is no comprehensive study addressing autophagy‐related genes expression status in AML . As a critical mediator, BECN 1 influences the onset and advance of autophagy and several studies have pointed to the BECN 1 recurrent allelic deletion and expression variation in a broad range of tumors. To explore this caveat, we chose this alteration‐prone gene to investigate in our study. Methods We have analyzed the expression status of BECN 1 in a series of 128 de novo AML patients using real‐time quantitative polymerase chain reaction ( qRT ‐ PCR ). Results In our favorable subgroup, BECN 1 expression did not alter ( P = 0.301), but in intermediate and unfavorable patients, we have had BECN 1 low expression compared to the normal controls ( P = 0.008 and P < 0.001, respectively). We found evidence for the association of reduced expression of BECN 1 with FLT 3‐ ITD mutation (19 of 27 patients), monosomal karyotype (all of 11 patients), higher age, and WBC count. Conclusion Overall, remarkable association of reduced expression of BECN 1 with FLT 3‐ ITD mutation and monosomal karyotype and their functional relationship is interesting which should be addressed and verified in future studies.