Premium
Flow cytometry in hematological nonmalignant disorders
Author(s) -
Béné M. C.,
Le Bris Y.,
Robillard N.,
Wuillème S.,
Fouassier M.,
Eveillard M.
Publication year - 2016
Publication title -
international journal of laboratory hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.705
H-Index - 55
eISSN - 1751-553X
pISSN - 1751-5521
DOI - 10.1111/ijlh.12438
Subject(s) - hypereosinophilia , paroxysmal nocturnal hemoglobinuria , hematology , flow cytometry , medicine , pathology , lymphoproliferative disorders , hematological disorders , peripheral blood , platelet , immunology , lymphoma , eosinophilia
Summary Multiparameter flow cytometry ( MFC ) has become an integral part of the diagnosis and classification of hematological malignancies. However, several nonmalignant or premalignant disorders may benefit from this technology in hematology laboratories. This review provides information on the normal immunophenotypic characteristics of peripheral blood leukocyte subsets and their modifications in several clinical conditions. The usefulness of MFC and the specific markers that can be investigated in hyperlymphocytosis, infection, hypereosinophilia, paroxysmal nocturnal hemoglobinuria, and large granular lymphocyte disorders is described. Mention is also made of the developments of MFC for analyses of red blood cells or platelets.