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ICSH recommendations for assessing automated high‐performance liquid chromatography and capillary electrophoresis equipment for the quantitation of HbA 2
Author(s) -
Stephens A. D.,
Colah R.,
Fucharoen S.,
Hoyer J.,
Keren D.,
McFarlane A.,
Perrett D.,
Wild B. J.
Publication year - 2015
Publication title -
international journal of laboratory hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.705
H-Index - 55
eISSN - 1751-553X
pISSN - 1751-5521
DOI - 10.1111/ijlh.12413
Subject(s) - capillary electrophoresis , chromatography , chemistry , electrophoresis
Summary Automated high performance liquid chromatography and Capillary electrophoresis are used to quantitate the proportion of Hemoglobin A 2 (HbA 2 ) in blood samples order to enable screening and diagnosis of carriers of β‐thalassemia. Since there is only a very small difference in HbA 2 levels between people who are carriers and people who are not carriers such analyses need to be both precise and accurate. This paper examines the different parameters of such equipment and discusses how they should be assessed.