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Detection of malignant cells in serous body fluids by counting high‐fluorescent cells on the Sysmex XN‐2000 hematology analyzer
Author(s) -
Labaere D.,
Boeckx N.,
Geerts I.,
Moens M.,
Van den Driessche M.
Publication year - 2015
Publication title -
international journal of laboratory hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.705
H-Index - 55
eISSN - 1751-553X
pISSN - 1751-5521
DOI - 10.1111/ijlh.12393
Subject(s) - hematology analyzer , serous fluid , body fluid , pathology , medicine , malignancy , hematology , nuclear medicine , chemistry
Summary Introduction The body fluid mode of the Sysmex XN‐2000 hematology analyzer differentiates cells into mononuclear and polymorphonuclear white blood cells (WBC) and high‐fluorescent cells (HFC). The aim of this study was to evaluate the performance of the HFC count for detecting malignant cells in serous body fluids. Methods Two‐hundred and thirty serous fluids were analyzed on the Sysmex XN body fluid mode. HFC were measured as relative count (HFC/100 WBC) and absolute count (HFC/ μ L). All samples were microscopically screened on cytospin slides for the presence of malignant cells. Results Malignant cells were found by microscopic examination in 49 of 230 samples (21.3%). Malignant samples contained significantly higher percentages (10.2 vs . 2.6/100 WBC) and absolute numbers (65 vs . 10/ μ L) of HFC than nonmalignant samples ( P < 0.001). Areas under the ROC curve for relative and absolute HFC count were 0.69 and 0.77, respectively. A cutoff level of ≥17 HFC/ μ L showed the best performance to predict malignancy, with 88% sensitivity and 61% specificity. Conclusion As serous body fluids will be more analyzed on automated analyzers in the future, HFC count can be a useful tool to select samples for microscopic review. Microscopic evaluation should be performed if HFC values are above a certain threshold (e.g. ≥17 HFC/ μ L) or in case of clinical suspicion of malignancy.