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Body fluid cellular analysis using the Sysmex XN ‐2000 automatic hematology analyzer: focusing on malignant samples
Author(s) -
Cho Y.U.,
Chi H.S.,
Park S. H.,
Jang S.,
Kim Y.J.,
Park C.J.
Publication year - 2015
Publication title -
international journal of laboratory hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.705
H-Index - 55
eISSN - 1751-553X
pISSN - 1751-5521
DOI - 10.1111/ijlh.12292
Subject(s) - hematology analyzer , receiver operating characteristic , body fluid , cell counting , cerebrospinal fluid , nuclear medicine , cutoff , hematology , medicine , pathology , chemistry , cancer , physics , quantum mechanics , cell cycle
Summary Introduction The majority of previous studies on body fluid ( BF ) mode of automatic hematology analyzer used nonmalignant BF samples. Here, we evaluated the BF mode on the recently launched Sysmex XN for counting blood cells, especially for malignant samples. Methods A total of 405 BF specimens including 125 malignant samples were analyzed using both the automated method and manual microscopy. Results In non‐cerebrospinal fluids ( CSF ) samples, there was an agreement between two methods for WBC , RBC , polymorphonuclear, and mononuclear cell counts ( R 2 = 0.96, 0.94, 0.88, and 0.88, respectively). CSF samples showed slightly poorer correlations than other fluids. Exclusion of malignant samples significantly improved correlations in non‐ CSF samples, but not in CSF samples. High fluorescence‐ BF ( HF ‐ BF ) cells were identified significantly more frequently in malignant samples compared to benign samples (17.8 and 4.15/100 WBC , respectively; P < 0.001). Receiver operating characteristic curve analysis demonstrated an HF ‐ BF cell AUC of 0.791 using a cutoff value of 6.9/100 WBC for detecting malignant samples. Conclusion The BF mode on the Sysmex XN could be an alternative method for the manual counts in the BF analysis with a few drawbacks. However, if a concentration of HF ‐ BF cells is greater than the given threshold, microscopic examination should be subsequently performed.