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Clinical relevance of CD 157 for rapid and cost‐effective simultaneous evaluation of PNH granulocytes and monocytes by flow cytometry
Author(s) -
Marinov I.,
Kohoutová M.,
Tkáčová V.,
Pešek A.,
Čermák J.,
Cetkovský P.
Publication year - 2015
Publication title -
international journal of laboratory hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.705
H-Index - 55
eISSN - 1751-553X
pISSN - 1751-5521
DOI - 10.1111/ijlh.12271
Subject(s) - coefficient of variation , flow cytometry , cytometry , wilcoxon signed rank test , reproducibility , paroxysmal nocturnal hemoglobinuria , limits of agreement , medicine , mann–whitney u test , chemistry , immunology , chromatography , nuclear medicine
Summary Introduction CD 157 has been reported as a potentially useful marker for paroxysmal nocturnal hemoglobinuria ( PNH ) testing by flow cytometry ( FCM ). Methods We determined the performance characteristics of a CD 157‐based five‐color assay and compared results from patient analysis with results obtained with a previously validated CD 14/ CD 24‐based six‐color protocol. Results Coefficient of variation ( CV ) for intra‐/interassay precision analysis of granulocytes ranged from 0.88/0.09% to 3.02/3.71% and from 0.20/0.08% to 8.83/4.04% for the five‐ and six‐color protocol, respectively. For monocyte, CV ranged from 0.42/0.49% to 8.13/4.80% for the five‐color protocol and from 0.28/0.70% to 5.41/3.19% for the six‐color protocol within various PNH clones. Coefficient of determination ( r 2 ) for linear regression analysis of PNH clones ranging from 0.3 to 99.8% was >0.99 in all cases, Wilcoxon ranks test showed no statistically significant differences ( P > 0.05), and Bland–Altman analysis demonstrated agreement with mean bias ranging from −0.02 to 0.38. Conclusion Our results confirm very good performance characteristics for both intra‐ and interassay precision analyses, excellent correlation, and agreement between approaches. In agreement with recently published data, our experience supports the clinical relevance of CD 157 for a rapid and cost‐effective simultaneous evaluation of PNH leukocytes by flow cytometry.