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Novel and rapid enumeration method of peripheral blood stem cells using automated hematology analyzer
Author(s) -
Tanosaki R.,
Kumazawa T.,
Yoshida A.,
Oguni S.,
Nakano A.,
Yamagata S.,
Takahashi N.,
Kurosawa S.,
Kim S. W.,
Yamashita T.,
Mori S.,
Heike Y.,
Fukuda T.,
Hamaguchi Y.,
Tsuda H.
Publication year - 2014
Publication title -
international journal of laboratory hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.705
H-Index - 55
eISSN - 1751-553X
pISSN - 1751-5521
DOI - 10.1111/ijlh.12182
Subject(s) - apheresis , hematology analyzer , haematopoiesis , leukapheresis , stem cell , hematology , enumeration , progenitor cell , cd34 , flow cytometry , peripheral blood , cytometry , hemolysis , immunology , transplantation , medicine , biology , platelet , mathematics , microbiology and biotechnology , combinatorics
Summary Introduction The number of infused CD 34 + cells is crucial to the success of peripheral blood stem cell transplantation ( PBSCT ). Here, we present, for the first time, a new method of enumerating hematopoietic progenitor cells ( HPC s) for PBSCT . Method This novel method is based on hemolysis and chemical staining, followed by flow cytometry‐based optical detection, conducted using an automated hematology analyzer ( XN series, Sysmex). CD 34 + cells and HPC s were compared in 76 granulocyte colony‐stimulating factor (G‐ CSF )‐mobilized blood or apheresis samples taken from healthy donors ( n = 18) or patients undergoing autologous PBSCT ( n = 6). Results There was a strong correlation between the numbers of HPC s and CD 34 + cells ( R 2 = 0.958). The expected total number of HPC s in the final products, which was estimated from HPC s in pre‐apheresis PB or mid‐apheresis products, also correlated well with the total number of CD 34 + cells in the final products. The change in HPC s in PB closely resembled that of CD 34 + cells during mobilization. Experiments using immunomagnetic beads suggested that the majority of CD 34 + cells existed in HPC s, and vice versa. Conclusion Hematopoietic progenitor cells may serve as surrogates for CD 34 + cells in PBSCT . However, further investigations are required to verify this.