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Evaluation of the S ysmex XT ‐4000 i for the automated body fluid analysis
Author(s) -
Lehto T. M.,
Leskinen P.,
Hedberg P.,
Vaskivuo T. E.
Publication year - 2014
Publication title -
international journal of laboratory hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.705
H-Index - 55
eISSN - 1751-553X
pISSN - 1751-5521
DOI - 10.1111/ijlh.12134
Subject(s) - cell counting , white blood cell , body fluid , automated method , chromatography , chemistry , medicine , cell , pathology , immunology , computer science , artificial intelligence , biochemistry , cell cycle
Summary Introduction Body fluid ( BF ) analysis is traditionally performed using microscopy, which is time‐consuming and labor‐intensive and has wide interobserver variability. Automation has been suggested to improve these elements. Methods Body fluid mode of the S ysmex XT ‐4000 i was used to analyze 51 cerebrospinal fluid ( CSF ) and 81 BF samples. white blood cell ( WBC ) count (cell/μL), WBC differential ( MN % and PMN %), and turnaround time ( TAT ) were compared with manually performed microscopic counting. Results Within‐run imprecision of BF ( CV %) was 2.8–19.3. Functional sensitivity was 18 cell/μL ( CV % 19.3). The accuracy of WBC count was moderate with CSF samples ( r s = 0.55) and good with BF samples ( r s = 0.95). The TAT of BF and CSF samples decreased with samples without high HF ‐ BF count when analyzed with XT ‐4000 i . Conclusion The BF mode of Sysmex XT ‐4000 i was found to be accurate and precise with samples where the WBC count was >200 cell/μL. Our results indicate that Sysmex XT ‐4000 i can help to reduce TAT of BF sample analysis and decrease work effort in a laboratory. However, automatically performed cell counting cannot replace microscopic analysis in samples with abnormal findings.