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Evaluation of ABX P entra DX 120 and S ysmex XE ‐2100 in umbilical cord blood
Author(s) -
Kim H.,
Hur M.,
Choi S.G.,
Kim B.H.,
Moon H.W.,
Yun Y.M.,
Hwang H. S.,
Kwon H. S.,
Sohn I. S.
Publication year - 2013
Publication title -
international journal of laboratory hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.705
H-Index - 55
eISSN - 1751-553X
pISSN - 1751-5521
DOI - 10.1111/ijlh.12110
Subject(s) - abx test , chemistry , cord blood , peripheral blood mononuclear cell , umbilical cord , haematopoiesis , flow cytometry , andrology , microbiology and biotechnology , stem cell , medicine , immunology , biochemistry , biology , mathematics , statistics , in vitro
Summary Introduction Cord blood ( CB ) is an important source of hematopoietic stem cells and reflects the hematologic status of neonates. ABX P entra DX 120 ( H oriba M edical, M ontpellier, F rance) and S ysmex XE ‐2100 ( S ysmex, K obe, J apan) were compared in 200 CB specimens. Methods Complete blood count parameters including white blood cell ( WBC ) differential counts were compared between the two analyzers. Double differential matrix ( DDX ) by ABX P entra DX 120 and hematopoietic progenitor cell ( HPC ) by S ysmex XE ‐2100 were compared with CD 34 + cells by flow cytometry. Results Most of the parameters showed acceptable correlation between the two analyzers. Although WBC differential of both analyzers showed acceptable correlation with manual counts, mononuclear cells ( MNC ) by ABX Pentra DX 120 better correlated with manual count than MNC by S ysmex XE ‐2100. NRBC by S ysmex XE ‐2100 better correlated with manual count than NRBC by ABX P entra DX 120. ABX Pentra DX 120 showed better flagging performances. DDX better correlated with CD 34 + cells than HPC . Conclusion Although the results from both analyzers are mostly interchangeable and reliable in CB specimens, flagging performance of ABX P entra DX 120 seems to be superior to that of S ysmex XE ‐2100. DDX by ABX Pentra DX 120 would be valuable to evaluate the quality of CB for further therapeutic utilization.