Evaluation of amotosalem treated platelets over 7 days of storage with an automated cytometry assay panel

Summary Introduction Pathogen Inactivation allows to overcome microbial contamination and growth related to storage of platelets concentrates ( PC ) at room temperature. The aim of our study was to evaluate the platelet storage lesion extending the storage period of pathogen inactivated platelet concentrates over 7 days using an automated cytometry assay panel. Methods We analyzed 43 concentrates subjected to pathogen inactivation ( CPPI ) at 3, 5 and 7 days evaluating: platelet count, mean platelet volume, platelets at low optical density, platelets at high density, GPII b‐ III a glycoprotein, platelet microparticles, lactate dehydrogenase. The collection bags (Fenwal) and the IBS kit made in PL 2410/ PL 2411 are approved for the conservation of PC up to 7 days. Data analysis was performed with anova test. Results All the parameters except small platelets and PMP were statistically different among day 7 vs . 3 and day 7 vs . 5. Conclusions Our study showed a progressive modification of pathogen inactivated platelet concentrates observed up to 7 days. The persistence of the secretory pool and the presence of the platelet membrane fibrinogen receptor suggest the persistence of a potential hemostatic efficacy. Clinical studies are necessary to directly correlate this type of analysis to 24 h recovery or survival of transfused platelets in humans.