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Immunophenotyping of selected hematologic disorders – focus on lymphoproliferative disorders with more than one malignant cell population
Author(s) -
Porwit A.
Publication year - 2013
Publication title -
international journal of laboratory hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.705
H-Index - 55
eISSN - 1751-553X
pISSN - 1751-5521
DOI - 10.1111/ijlh.12080
Subject(s) - immunophenotyping , lymphoproliferative disorders , population , cd5 , cd19 , immunology , cytometry , cd20 , medicine , flow cytometry , antigen , lymphoma , environmental health
Summary Currently, clinical laboratories face increasing demand for flow cytometry testing combined with limited funding. Therefore, many laboratories search for panels that would provide sufficient immunophenotyping information and meet economical requirements. At the Flow Cytometry Laboratory, University Health Network, Toronto, ON , Canada, we apply two 10‐color tubes of surface markers for diagnosis of lymphoproliferative disorders ( LPD s). These tubes contain most of the mandatory B ‐ and T ‐cell markers according to European Leukemia Net ( www.leukemia-net.org ) recommendations. The B‐cell‐oriented panel includes the following antibodies: Kappa‐ FITC /lambda‐ PE / CD 19‐ ECD / CD 38‐ PC 5.5/ CD 20‐ PC 7/ CD 34‐ APC / CD 23 APC ‐ AF 700/ CD 10 APC ‐ AF 750/ CD 5‐ PB / CD 45‐ KO . A different combination is applied to detect cytoplasmic Ig light chain expression and aberrant immunophenotype of plasma cells. The T‐cell panel allows enumeration of various T ‐ and NK ‐cell subsets: CD 57‐ FITC / CD 11c‐ PE / CD 8‐ ECD / CD 3‐ PC 5.5/ CD 2‐ PC 7/ CD 56‐ APC / CD 7‐ APC ‐ AF 700/ CD 4‐ APC ‐ AF 750/ CD 5‐ PB / CD 45‐ KO . The reported overall incidence of B ‐cell chronic LPD s presenting with more than one aberrant population is approximately 5%. Multicolor analysis facilitates the detection of multiple aberrant populations in the same sample because expression of multiple antigens can be studied simultaneously in each defined population. Examples of LPD s with multiple aberrant populations are presented.