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Pertinence of the S ysmex XE ‐5000™ parameters: rule of slide review in a context of ‘normal’ lymphocyte count (defined from control and mantle cell lymphoma blood specimens)
Author(s) -
Chhuy J.,
Morel D.,
Goeddert G.,
Magaud J.P.,
Felman P.,
Baseggio L.
Publication year - 2013
Publication title -
international journal of laboratory hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.705
H-Index - 55
eISSN - 1751-553X
pISSN - 1751-5521
DOI - 10.1111/ijlh.12042
Subject(s) - context (archaeology) , lymphocyte , mantle cell lymphoma , hematology analyzer , receiver operating characteristic , lymphoma , pathology , medicine , spectrum analyzer , computer science , biology , paleontology , telecommunications
Summary Introduction As most hematology cell analyzers, the different parameters of Sysmex XE ‐5000™ are little informative in the qualitative analysis of lymphoid cells, and especially when the lymphocyte count is below 4 × 10 9 /L (i.e., ‘normal’). The aim of our study was to investigate whether some parameters and/or ‘flags’ not routinely provided by this analyzer, but reachable by operator could be reliable to define rules of slide review in absence of common qualitative and quantitative alarms particularly in case of ‘normal’ lymphocyte count. Methods Blood samples from 13 mantle cell lymphoma fully annotated cases, and 180 control specimens were studied with S ysmex XE ‐5000™ analyzer. All cases did not present any anomalies in common quantitative and structural parameters. Results Using the method of area under the curve and ROC curve analysis, we described a novel threshold of alarm VAL _ ABN LYMPH (≥40 instead of 100 defined by S ysmex), as well as a pertinent L y X threshold (≥89). The combination of these thresholds allowed defining a rule of slide review in context of ‘normal’ lymphocyte count. Conclusion Among the parameters provided by the S ysmex XE ‐5000™ analyzer, the combination of the alarm VAL _ ABN LYMPH and the L y X value, routinely available on a simple blood analysis appears particularly informative to trigger slide review in a context of ‘normal’ lymphocyte count with a good sensitivity (85%) to detect circulating lymphoma cells and with <1% of false positive results.