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Utility of new mature erythrocyte and reticulocyte indices in screening for iron‐deficiency anemia in a pediatric population
Author(s) -
Osta V.,
Caldirola M. S.,
Fernandez M.,
Marcone M. I.,
Tissera G.,
Pennesi S.,
Ayuso C.
Publication year - 2013
Publication title -
international journal of laboratory hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.705
H-Index - 55
eISSN - 1751-553X
pISSN - 1751-5521
DOI - 10.1111/ijlh.12030
Subject(s) - medicine , anemia , erythropoiesis , hemoglobin , reticulocyte , population , cutoff , receiver operating characteristic , iron deficiency , gastroenterology , biology , physics , biochemistry , environmental health , messenger rna , gene , quantum mechanics
Summary Introduction Iron‐deficient erythropoiesis attributable to nutritional deficiency is the most common cause of anemia in early childhood. Beckman Coulter has proposed new parameters: red blood cell size factor ( RS f) and low hemoglobin density ( LHD %). RSf = MCV × MRVLHD % = 100 1 ‐ [ 1 / ( 1 + e1 . 8 ( 30 ‐ MCHC )) ]The aims of the present communication were to establish reference ranges ( RR ) for RS f and LHD % in a healthy pediatric population; to evaluate clinical utility of these parameters in the diagnosis of iron‐deficiency anemia ( IDA ); and to compare RS f and LHD % with RET ‐He. Methods Two hundred healthy infants and 51 with IDA were analyzed on Coulter LH 750 and Sysmex XT 2000i analyzers. Results RR for RS f in children aged 0.5–6 years, 82.2–102.0 and 83.7–103.1 fl in the group aged 6‐18 years. RR for LHD % was 1.1–9.0%. ROC analysis for RS f and LHD % in the diagnosis of IDA showed an AUC 0.8460 and 0.8654, respectively. The best RS f value to detect a restricted erythropoiesis was 89fl, sensitivity 92%, specificity 81%. LHD % optimal cutoff point was 6.0%, sensitivity 80%, and specificity 88%. Good correlation was observed between these parameters with Ret‐He, r = 0.888 and r = −0.790, respectively. Conclusion RS f and LHD % could be useful tools in the screening of IDA . These parameters can be obtained in the course of routine blood counts, with no additional cost or need for more blood sampling.