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Performance of a novel BD Stem Cell Enumeration Kit on two flow cytometry systems
Author(s) -
OmanaZapata I.,
Oreizy F.,
Mosqueda F.,
Salemenik M.,
Lin A.,
Wen J. L.,
Varro R.,
LeeHaynes L.,
Segurado O. G.
Publication year - 2013
Publication title -
international journal of laboratory hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.705
H-Index - 55
eISSN - 1751-553X
pISSN - 1751-5521
DOI - 10.1111/ijlh.12029
Subject(s) - enumeration , flow cytometry , stem cell , computational biology , biology , immunology , chemistry , computer science , microbiology and biotechnology , mathematics , combinatorics
Summary Introduction Hematopoietic stem cell transplantation, which requires accurate enumeration of stem cells, is routinely used in clinical settings. Flow cytometry provides a qualitative and quantitative assessment of CD 34 + cells. Precision, linearity, and stability of the novel BD ™ S tem C ell E numeration ( SCE ) K it were evaluated on two flow cytometry platforms using a modified ISHAGE gating strategy and including a viability dye for data acquisition and analysis. Methods Precision and linearity were evaluated on BD FACSC anto™ II and BD FACSC alibur™ systems. Stability was evaluated on the BD FACSC anto II system. Precision was tested using both high and low controls. Linearity was evaluated using dilutions from CD 34 + cell pools, while stability was evaluated using fresh leukapheresis specimens. Results Both systems showed precision with limited variability in absolute counts and percentages of viable CD 34 + cells. The linearity range of viable CD 34 + cells in both systems was established at 0–1000 cells/μL, showing a linear relationship ( R 2 = 0.99). Stability of CD 34 + cells in mobilized leukapheresis samples was confirmed up to 24 h after collection and up to 60 min after the end of stain/lyse procedures. Conclusion The BD SCE Kit on both flow cytometry systems shows consistent and reproducible results.