Premium
Effects of cell salvage on erythrocyte 2,3‐disphosphoglycerate and G‐6‐ PD levels and phosphatidylserine expression
Author(s) -
Che J.,
Tian M.,
Ding G.,
Huai Q.,
Dong P.,
Li Y.,
Li S.
Publication year - 2013
Publication title -
international journal of laboratory hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.705
H-Index - 55
eISSN - 1751-553X
pISSN - 1751-5521
DOI - 10.1111/ijlh.12028
Subject(s) - venous blood , autotransfusion , red blood cell , medicine , perioperative , phosphatidylserine , chemistry , anesthesia , biochemistry , phospholipid , blood transfusion , membrane
Summary Introduction The aim of this study was to investigate the contribution of the perioperative cell salvage process to the changes in erythrocyte 2,3‐disphosphoglycerate (2,3‐ DPG ) and glucose‐6‐phosphate dehydrogenase ( G ‐6‐ PD ) levels and phosphatidylserine ( PS ) expression using a self‐control study comparing washed blood with venous blood.Methods Thirty patients undergoing elective heart and blood vessel surgery were enrolled. Blood was collected and processed using a Fresenius continuous autotransfusion system device. 2,3‐ DPG and G ‐6‐ PD activities, as well as PS expression, from both venous and washed red blood cells ( RBC ) were measured. We also compared these indicators among washed RBC at 6 h, washed RBC at 0 h, and venous RBC .Results 2,3‐ DPG and G ‐6‐ PD activities in washed blood at 0 h were significantly higher than in venous RBC (2,3‐ DPG , venous vs. washed blood P < 0.05 ; G ‐6‐ PD , venous vs. washed blood P < 0.05 ). Flow cytometry results showed no differences between venous blood and washed RBC at 0 h ( n = 23, P > 0.05). 2,3‐ DPG activity in washed RBC that were allowed to stand for 6 h decreased compared with that in venous blood ( venous vs. washed blood at 6 h P < 0.01 ), whereas no significant difference was observed in G ‐6‐ PD activity ( venous. vs. washed blood at 6 h P > 0.05 ).Conclusion Compared with venous RBC , washed RBC from intraoperative cell salvage exhibited good oxygen‐carrying and antioxidant capacities. However, the oxygen‐carrying capacity of washed RBC that were allowed to stand for 6 h was not as good as that of venous RBC . Thus, we suggest that washed RBC that were left to stand for more than 6 h should not be reinfused.