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Automated, semi‐automated, and manual analyses of anti‐cardiolipin and anti‐β2‐glycoprotein I antibodies in women with a history of miscarriage
Author(s) -
Gutensohn K.,
Vossen D.,
Strate A.,
Kersten J. F.,
Hofbauer M.,
Krieger T.
Publication year - 2013
Publication title -
international journal of laboratory hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.705
H-Index - 55
eISSN - 1751-553X
pISSN - 1751-5521
DOI - 10.1111/ijlh.12011
Subject(s) - cardiolipin , antibody , miscarriage , glycoprotein , medicine , gynecology , immunology , pregnancy , chemistry , biology , microbiology and biotechnology , phospholipid , biochemistry , membrane , genetics
Summary Introduction Anti‐cardiolipin and β2‐glycoprotein I antibodies represent important diagnostic parameters in routine hematology. In this study, five different automated, semi‐automated, and manual immunoassays detecting IgG/IgM anti‐cardiolipin and anti‐β 2 ‐glycoprotein I antibodies were tested. Methods A total of 162 samples from women with a history of miscarriage were recruited from 110 different G&O outpatient centers in Germany. Results For both anti‐cardiolipin and anti‐β 2 ‐glycoprotein I antibodies, considerable differences in the percentage of positive results were seen between all five methods, and itemization of all positive test results revealed a poor accordance. These findings were confirmed by Cohen's kappa coefficients. Conclusion Our study revealed a moderate to poor accordance between five different test systems for anti‐cardiolipin and anti‐β 2 ‐glycoprotein I antibodies. Such deviations may result in clinical misinterpretation of data and may lead to wrong therapeutic consequences. Therefore, further standardization of all tests for anti‐phospholipid antibodies should be achieved.