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Characterization of a novel allelic variant in HLA‐B*46:01 lineage, HLA‐B*46:01:25 , by cloning, phasing and sequencing
Author(s) -
Li LiXin,
Tian Wei,
Zhu FaMing,
Wang WenYi,
Wang Fan
Publication year - 2018
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/iji.12392
Subject(s) - genetics , biology , human leukocyte antigen , allele , lineage (genetic) , exon , gene , polymerase chain reaction , hla b , microbiology and biotechnology , cloning (programming) , antigen , computer science , programming language
A new allelic variant in HLA‐B*46:01 lineage, HLA‐B*46:01:25, has been identified in a male individual of Mongol ethnicity residing in northern China. Following polymerase chain reaction‐sequence‐based typing (PCR‐SBT), this novel variant was further confirmed by cloning, phasing and sequencing. HLA‐B*46:01:25 differs from HLA‐B*46:01:01 by a single synonymous T substitution at nucleotide position 137 (C ‐ T) in exon 4, corresponding to codon 228 (ACC‐ACT) of the mature HLA‐B mRNA molecule.