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Characterization of a novel allelic variant in HLA ‐B*40 lineage, HLA ‐B*40:298:02, by cloning and sequencing
Author(s) -
Tian W.,
Zhu F. M.,
Wang W. Y.,
Li L. X.,
Cai J. H.
Publication year - 2018
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/iji.12372
Subject(s) - cloning (programming) , allele , genetics , human leukocyte antigen , biology , gene , antigen , computer science , programming language
Summary A novel allelic variant in HLA ‐B*40 lineage, HLA ‐ B*40:298:02 , has been identified in an individual of Han ethnicity afflicted with nasopharyngeal carcinoma in Hunan province, southern China. Following polymerase chain reaction–Sanger sequence‐based typing ( PCR – SBT ), this new variant was further confirmed by two distinct strategies of cloning and sequencing. HLA ‐ B*40:298:02 differs from HLA ‐B*40:298:01 by a single synonymous cytosine substitution at nucleotide position 26 (T→C) in exon 3, which corresponds to codon 99 of the mature HLA ‐B mRNA molecule. This new allele has an estimated frequency of 0.0002, in about 2,500 sequence‐based typed subjects from the same population.