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Association of CD14 and macrophage migration inhibitory factor gene polymorphisms with inflammatory microRNAs expression levels in ankylosing spondylitis and polyarthralgia
Author(s) -
Vishwakarma S. K.,
Lakkireddy C.,
Sravani G.,
Sastry B. V. S.,
Raju N.,
Ahmed S. I.,
Khan A. A.,
Owaisi N.,
Jaisawal A.,
Khan M. A.,
Khan A. A.
Publication year - 2018
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/iji.12366
Subject(s) - macrophage migration inhibitory factor , genotype , ankylosing spondylitis , genotyping , immunology , single nucleotide polymorphism , genetic predisposition , gene expression , biology , rna , inflammation , gene , medicine , genetics , cytokine
Summary This study aimed to investigate the genetic basis of ankylosing spondylitis ( AS ) and polyarthralgia ( PA ) conditions among Indian subjects through genotyping two immune regulatory genes CD 14 (−159C>T) and MIF (−173G>C) and find their association with the expression levels of three circulating inflammatory mi RNA s. This investigation may provide early genetic cause of these two forms of arthritis and more optimal biological targets to predict early therapeutic outcomes. A total of 140 patients ( AS : 70 and PA : 70) and 156 controls were recruited from Indian population. CD 14 and MIF genotyping was performed using ARMS – PCR . Expression level of three inflammatory mi RNA s (mi RNA ‐146a, mi RNA ‐155 and mi RNA ‐181) was quantified using RT – qPCR . C/T genotype of CD 14 gene was found to cause 2.06‐fold risk of developing AS ( CI 1.06–5.98, p = .04) as compared to others and G/C genotype in MIF also shown significant variation between AS and control subjects. In PA subjects, CD 14 genotypes (C/T) was found to be associated with disease susceptibility and G/C genotype of MIF gene polymorphism showed 4.71‐fold risk of developing PA ( CI 2.58–8.62, p = .0001). The study also revealed significant upregulation of mi RNA ‐155 expression in AS subjects ( p = .0001) with more than 1.3‐fold difference between AS and PA as compared to the control subjects. mi RNA ‐155 had strong association with AS patients with CD 14 genotypes ( p < .05) than PA and control subjects. This study provides better understanding of the mechanisms and disease susceptibility for MIF and CD 14 genetic variants and inflammatory mi RNA s networks involved in AS and PA .