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Genetic polymorphism of human leucocyte antigen and susceptibility to multidrug‐resistant and rifampicin‐resistant tuberculosis in Han Chinese from Hubei Province
Author(s) -
Zhou X.,
Zhou Q.,
Yang Z.F.,
Li W.X.
Publication year - 2018
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/iji.12352
Subject(s) - tuberculosis , genotyping , haplotype , allele , human leukocyte antigen , immunology , biology , population , genotype , mycobacterium tuberculosis , medicine , genetics , virology , antigen , gene , pathology , environmental health
Summary We determined the high‐resolution allele and haplotype frequencies at the human leucocyte antigen ( HLA )A, B and DRB 1 loci in the Han population of Hubei province, the TB endemic area of Central China, with pulmonary tuberculosis ( PTB ), and established the relationship between HLA ‐A, B and DRB 1 alleles as well as haplotypes and susceptibility to multidrug‐resistant and rifampicin‐resistant tuberculosis ( MDR / RR ‐ TB ). Blood samples were drawn from 174 patients with MDR / RR ‐ TB and 838 patients with drug‐susceptible PTB in ethnic Han population from Hubei province (central China). Four‐digit allele genotyping of HLA‐ A, B and DRB1 loci was performed using polymerase chain reaction with sequence‐specific oligonucleotide probes (PCR‐ SSOP). The allele and haplotype frequencies of HLA ‐A, B and DRB 1 were determined and compared between patients with MDR / RR ‐ TB and patients with drug‐susceptible PTB . Statistical analysis of the generated data indicated no departure from expectation of Hardy–Weinberg equilibrium ( HWE ) at all loci of the control group. Multivariate analysis identified allele DRB 1*08:01 ( p < .0001; OR = 174.5, 95% CI 15.3–1987.2) as independent predictor of MDR / RR ‐ TB , except for old age ( p < .0001; OR = 10. 9, 95% CI 7.6–15.8), previous treatment history ( p < .0001; OR = 11.0, 95% CI 7.2–16.7) and poor compliance to treatment ( p < .0001; OR = 12.9, 95% CI 8.4–20.0). While in the subgroup of new TB cases, DRB 1*08:01 ( p < .0001; OR = 80.3, 95% CI 7.0–917.1) and older age ( p < .0001; OR = 3.9, 95% CI 2.4–6.4) were independent susceptibility factors for primary MDR / RR ‐ TB . Our results suggest that a combination of clinical and host genetic information about tuberculosis patients may contribute to prediction and early detection of MDR / RR ‐ TB .