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Characterization of a novel HLA ‐B*39:01:01‐ related allele, HLA ‐B*39:130 , by cloning and phasing
Author(s) -
Li L. X.,
Tian W.,
Zhu F. M.,
Wang W. Y.,
Cai J. H.
Publication year - 2017
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/iji.12345
Subject(s) - nonsynonymous substitution , microbiology and biotechnology , biology , genetics , exon , sanger sequencing , human leukocyte antigen , cloning (programming) , allele , epitope , gene , dna sequencing , antigen , genome , computer science , programming language
Summary A novel HLA ‐B*39:01:01 ‐related variant, HLA ‐B*39:130 , has been identified in a normal individual of Han ethnicity in Hunan province, southern China. Following Sanger polymerase chain reaction–sequence‐based typing ( PCR ‐ SBT ), this new allele was further confirmed by cloning, phasing and sequencing. Aligned with HLA ‐B*39:01:01 , HLA ‐B*39:130 has a nonsynonymous thymine substitution at nucleotide position 94 in exon 4, resulting in amino acid change from threonine to isoleucine at codon 214 ( ACA → ATA ) of the mature HLA ‐B mRNA molecule.