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The IL 15 +96522 A>T functional polymorphism is related to the differentiation of Th17 cells and the severity of Hashimoto's disease
Author(s) -
Arakawa Y.,
Watanabe M.,
Takemura K.,
Inoue N.,
Hidaka Y.,
Iwatani Y.
Publication year - 2017
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/iji.12305
Subject(s) - genotype , pathogenesis , allele , snp , immunology , graves' disease , cytokine , medicine , polymorphism (computer science) , interleukin , disease , autoimmune disease , single nucleotide polymorphism , biology , gene , genetics
Summary To clarify the association between the genetic producibility of IL ‐15, a pro‐inflammatory cytokine, and the pathogenesis of autoimmune thyroid diseases ( AITD s), we genotyped +96522 A>T and +82889 A>G polymorphisms in the IL 15 gene using 127 patients with Hashimoto's disease ( HD ), including 55 patients with severe HD and 48 patients with mild HD ; 130 patients with Graves’ disease ( GD ), including 52 patients with intractable GD and 44 patients with GD in remission; and 79 healthy volunteers. Both the IL 15 +96522 A allele and AA genotype were more frequent in patients with severe HD than in those with mild HD . The serum levels of IL ‐15 were higher in individuals with the IL 15 +96522 AA genotype than in those with the T allele, and they were also higher in patients with severe HD than in those with mild HD . On the other hand, the mRNA levels of IL ‐15 were not significantly different among individuals with each genotype of both SNP s. After incubation with recombinant human IL ‐15, the proportions of Th17 cells in CD 4 + cells were increased, and those of Treg cells in CD 4 + cells were maintained. Our study indicates that the IL 15 +96522A/C polymorphism correlates with the severity of HD , most likely by increasing Th17 cells.