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Characterization of a novel MICA allele, MICA *012:05 , by cloning and sequencing
Author(s) -
Wang W. Y.,
Tian W.,
Wang F.,
Zhu F. M.,
Li L. X.
Publication year - 2016
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/iji.12275
Subject(s) - mica , cloning (programming) , allele , exon , haplotype , polymerase chain reaction , microbiology and biotechnology , genetics , biology , gene , paleontology , computer science , programming language
Summary A new MICA allelic variant, MICA *012:05 , has been identified in a Chinese Mongolian population. Following polymerase chain reaction–sequence‐based typing ( PCR ‐ SBT ), this new allele was further confirmed by cloning and sequencing. MICA *012:05 was linked to an HLA ‐A*24‐C*01‐B*55:02‐ DRB 1*09 haplotype. MICA *012:05 differs from MICA *012:01 by a single synonymous C to T substitution at nucleotide position 269 in exon 3.