Premium
HLA ‐A, HLA ‐B, HLA ‐ DRB 1 allele and haplotype frequencies of 14 529 Chinese Han bone marrow donors living in Dalian, China
Author(s) -
Shao L. N.,
Zhang S. T.,
Yu W. J.,
Zhou S. H.,
Duan Y.,
Pan L. Z.,
Wang N.,
Hu Y.
Publication year - 2016
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/iji.12248
Subject(s) - haplotype , allele , human leukocyte antigen , locus (genetics) , genetics , biology , hla a , linkage disequilibrium , population , allele frequency , immunology , gene , antigen , medicine , environmental health
Summary We investigated the allele and haplotype frequencies of HLA ‐A, HLA ‐B and HLA ‐ DRB 1 loci in Dalian Chinese Han population using blood samples of unrelated marrow donors who live in Dalian. The genetic relationship between Dalian and different regions worldwide was further explored based on HLA status of different populations. A total of 14 529 samples were genotyped at 2‐digit level only by sequence‐specific oligonucleotide and sequence‐based typing methods. Allele frequencies of HLA ‐A, HLA ‐B and HLA ‐ DRB 1 were calculated by the direct counting method. Haplotype frequencies and linkage disequilibrium ( LD ) values were calculated by the maximum likelihood method. F ST values were calculated by allele frequency data of each locus. Phylogeny tree of Nei's D A genetic distances was constructed by the UPGMA method. HLA ‐A*02 was the most frequent allele at HLA ‐A locus followed by A*11 and A*24. Alleles at HLA ‐B locus ranked in decreasing order by frequency were B*40, B*15 and B*13. The three highest frequency alleles were DRB 1*15, DRB 1*09 and DRB 1*12 at HLA ‐ DRB 1 locus. A*30‐B*13‐ DRB 1*07 was the most frequent three‐locus haplotype. For the population relationships, Dalian had a relative close genetic relationship with Liaoning and Yantai‐Weihai and a relative distant genetic relationship with Australia. The information obtained in this study may provide useful information for anthropological studies, for disease‐association studies and helping bone marrow transplantation patients to search HLA ‐matched donors.