Molecular characterization of classical and nonclassical MHC class I genes from the golden pheasant ( C hrysolophus pictus )

Summary Classical major histocompatibility complex ( MHC ) class I allelic polymorphism is essential for competent antigen presentation. To improve the genotyping efforts in the golden pheasant, it is necessary to differentiate more accurately between classical and nonclassical class I molecules. In our study, all MHC class I genes were isolated from one golden pheasant based on two overlapping PCR amplifications. In total, six full‐length class I nucleotide sequences (A–F) were identified, and four were novel. Two (A and C) belonged to the IA 1 gene, two (B and D) were alleles derived from the IA 2 gene through transgene amplification, and two (E and F) comprised a third novel locus, IA 3 that was excluded from the core region of the golden pheasant MHC ‐B. IA 1 and IA 2 exhibited the broad expression profiles characteristic of classical loci, while IA 3 showed no expression in multiple tissues and was therefore defined as a nonclassical gene. Phylogenetic analysis indicated that the three IA genes in the golden pheasant share a much closer evolutionary relationship than the corresponding sequences in other galliform species. This observation was consistent with high sequence similarity among them, which likely arises from the homogenizing effect of recombination. Our careful distinction between the classical and nonclassical MHC class I genes in the golden pheasant lays the foundation for developing locus‐specific genotyping and establishing a good molecular marker system of classical MHC I loci.