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Association study of interleukin‐1 family and interleukin‐6 gene single nucleotide polymorphisms in recurrent aphthous stomatitis
Author(s) -
Najafi S.,
Yousefi H.,
Mohammadzadeh M.,
Bidoki A. Z.,
Firouze Moqadam I.,
Farhadi E.,
Amirzargar A. A.,
Rezaei N.
Publication year - 2015
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/iji.12228
Subject(s) - recurrent aphthous stomatitis , single nucleotide polymorphism , genotype , interleukin , biology , allele , immunology , genetics , gene , microbiology and biotechnology , cytokine , medicine , stomatitis
Summary Recurrent aphthous stomatitis ( RAS ) is a common painful, ulcerative oral inflammatory disorder with unknown aetiology. Immune system and aberrant cytokine cascade deemed to be critical in outbreaks of RAS ulcers. Interleukin‐1 ( IL ‐1) and IL ‐6 are the most potent pro‐inflammatory cytokines. Single nucleotide polymorphisms ( SNP s) of IL ‐1 and IL ‐6 genes can affect the secretion of these cytokines. The aim of this study was to investigate the association between RAS and IL ‐6 and IL ‐1 in Iranian subjects with minor RAS . Genomic DNA was obtained from 64 Iranian patients with RAS . IL ‐1 α C −889 T, IL ‐1 β C −511 T, IL ‐1 β C +3962 T, IL ‐1R C pst‐I 1970 T, IL ‐1Ra C Mspa‐I11100 T, IL ‐6 C −174 G and IL ‐6 A nt +565 G polymorphisms were determined using polymerase chain reaction with sequence‐specific primers ( PCR ‐ SSP ). The frequency of C −174 C genotype in the patients group was significantly different from the healthy control. No other significant differences were found in genotype and alleles frequencies between the two groups. These results indicate that certain SNP s of IL ‐6 gene at position −174 which located in promoter have association with predisposition of individuals to RAS .