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HLA class II susceptibility pattern for type 1 diabetes (T1D) in an Iranian population
Author(s) -
Kiani J.,
Hajilooi M.,
Furst D.,
Rezaei H.,
ShahryariHesami S.,
Kowsarifard S.,
Zamani A.,
Solgi G.
Publication year - 2015
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/iji.12216
Subject(s) - haplotype , allele , heterozygote advantage , human leukocyte antigen , population , biology , genetics , type 1 diabetes , immunology , medicine , diabetes mellitus , antigen , gene , endocrinology , environmental health
Summary This study aimed to determine the HLA ‐ DRB 1/ HLA ‐ DQB 1 susceptibility and protection pattern for type 1 diabetes (T1D) in a population from Hamadan, north‐west of Iran. A total of 133 patients with T1D were tested for HLA ‐ DRB 1 and HLA ‐ DQB 1 alleles using PCR ‐ SSP compared to 100 ethnic‐matched healthy controls. Alleles and haplotypes frequencies were compared between both groups. The most susceptible alleles for disease were HLA ‐ DRB 1*03:01, DRB 1*04:02, DQB 1*02:01 and DQB 1*03:02, and protective alleles were HLA ‐ DRB 1*07:01, *11:01, *13:01, *14:01 and DRB 1*15 and HLA ‐ DQB 1*06:01, *06:02 and *06:03. Haplotype analysis revealed that patients with T1D had higher frequencies of DRB 1*03:01– DQB 1*02:01 ( OR = 4.86, P < 10 −7 ) and DRB 1*04:02– DQB 1*03:02 ( OR = 9.93, P < 10 −7 ) and lower frequencies of DRB 1*07:01– DQB 1*02:01 ( P = 0.0005), DRB 1*11:01– DQB 1*03:01 ( P = 0.001), DRB 1*13:01– DQB 1*06:03 ( P = 0.002) and DRB 1*15– DQB 1*06:01 ( P = 0.001) haplotypes compared to healthy controls. Heterozygote combination of both susceptible haplotypes ( DR 3/ DR 4) confers the highest risk for T1D ( RR = 18.80, P = 4 × 10 −5 ). Additionally, patients with homozygote diplotype, DR 3/ DR 3 and DR 4/ DR 4, showed a similar risk with less extent to heterozygote combination ( P = 0.0004 and P = 0.01, respectively). Our findings not only confirm earlier reports from Iranians but also are in line with Caucasians and partly with Asians and some African patients with T1D. Remarkable differences were the identification of DRB 1*04:01– DQB 1*03:02, DRB 1*07:01– DQB 1*03:03 and DRB 1*16– DQB 1*05:02 as neutral and DRB 1*13:01– DQB 1*06:03 as the most protective haplotypes in this study.