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Identification of a novel MICB allele, MICB *030 , by cloning and sequencing
Author(s) -
Wang W. Y.,
Wang F.,
Tian W.
Publication year - 2015
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/iji.12207
Subject(s) - nonsynonymous substitution , cloning (programming) , biology , polymerase chain reaction , exon , allele , genetics , typing , microbiology and biotechnology , gene , genome , computer science , programming language
Summary A novel MICB allele, MICB *030 , has been identified in a healthy Chinese individual of Mongol ethnicity residing in northern China by polymerase chain reaction sequence‐based typing ( PCR ‐ SBT ) and confirmed by cloning and sequencing. MICB *030 was linked to HLA ‐B*35 . Aligned with MICB *005:02 , MICB *030 has a nonsynonymous adenine substitution at nucleotide position 50 in exon 3, leading to amino acid change from serine to arginine at codon 102 of the mature MICB molecule.