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Allele and haplotype frequencies of HLA ‐A, B, C, DRB 1 and DQB 1 genes in polytransfused patients in ethnically diverse populations from Brazil
Author(s) -
Rodrigues C.,
Macedo L. C.,
Bruder A. V.,
Quintero F. d. C.,
Alencar J. B.,
Sell A. M.,
Visentainer J. E. L.
Publication year - 2015
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/iji.12206
Subject(s) - human leukocyte antigen , haplotype , genotyping , allele , typing , allele frequency , immunology , antigen , population , genetics , antibody , biology , hla a , ethnic group , gene , genotype , medicine , political science , law , environmental health
Summary The red blood transfusion is a practice often used in patients with haematological and oncological diseases. However, the investigation of human leucocyte antigen ( HLA ) system frequency in these individuals is of great importance because multiple transfusions may lead to HLA alloimmunization. Brazil is a country that was colonized by many other ethnicities, leading to a mixed ethnicity and regionalized population. In view of the importance of HLA typing in these patients, the aim of this study was to investigate the allele and haplotype frequencies from polytransfused patients from three different regions from Brazil. HLA ‐A, HLA ‐B, HLA ‐C, HLA ‐ DRB 1 and HLA ‐ DQB 1 genotyping of 366 patients was performed by PCR ‐ SSO , based on the Luminex technology (One Lambda ® ), and the anti‐ HLA class I and class II antibodies were analysed using LabScreen Single Antigen Antibody Detection (One Lambda, Inc.). Allele and haplotype frequencies of polytransfused patients of three regions from Brazil were obtained using the Arlequin program. The most frequent allele frequencies observed were HLA ‐A*02 , A*03 , B*15 , B*35 , B*51 , C*07 , C*04 , C*03 , DRB 1*13 , DRB 1*11 , DRB 1*07 , DRB 1*03 , DRB 1*01 , DQB 1*03 , DQB 1*02 , DQB 1*06 and DQB 1*05 . There were differences between the groups for allele variants HLA ‐B*57 (between Group 1 and Group 2) and HLA ‐C*12 (between Group 1 and Group 3). The most frequent haplotypes found in the sample were HLA ‐A*01B*08 DRB 1*03 , DRBI *07 DQB 1*02 , DRB 1*01 DQB 1*05 , DRB 1*13 DQB 1*06 and A* 02B*35 . HLA class I and II antibodies were detected in 77.9% and 63.9% patients, respectively, while the both alloantibodies were detected in 62 (50.9%) patients. In conclusion, the HLA typing for polytransfused patients in each region has a great importance, as seen in this study; individuals from different regions from Brazil have HLA distribution not completely homogeneous.