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Distribution of cytokine gene polymorphisms in five M alay subethnic groups in P eninsular M alaysia
Author(s) -
Norhalifah H. K.,
Zafarina Z.,
Sundararajulu P.,
Norazmi M. N.,
Edinur H. A.
Publication year - 2015
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/iji.12189
Subject(s) - genotyping , allele , cytokine , gene , primer (cosmetics) , single nucleotide polymorphism , biology , genetics , microbiology and biotechnology , genotype , chemistry , organic chemistry
Summary In this survey, we have successfully genotyped 22 single nucleotide polymorphisms in the 13 cytokine genes for five M alay subethnic groups ( K elantan, A cheh, M andailing, M inangkabau and P atani M alays) using polymerase chain reaction–sequence‐specific primer cytokine genotyping kit ( I nvitrogen, C arlsbad, CA , USA ). Most of the cytokine genes showed similar pattern of allelic spectra with wild‐type alleles (e.g. ILI a−889/ C , ILIB +3962/ C and IL6 nt565/ G ) that represent more than 80% in the studied M alay subethnic groups. These newly observed cytokine alleles and subsequent analyses clearly indicate genetic contribution from A sia in the studied M alay subethnic groups with evidence of admixture from neighbouring populations in P atani M alays. The cytokine data sets for the five M alay subethnic groups deposited in this report can also be used as reference standard for searching suitable donor for allograft transplant and diseases association study. This is particularly relevance as our analyses showed differences between the M alay subethnic groups and other populations screened for cytokine genes.