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A group‐specific sequence‐based typing approach for HLA ‐ DQA 1
Author(s) -
Lemin A. J.,
Johnson J.,
Darke C.
Publication year - 2015
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/iji.12171
Subject(s) - typing , human leukocyte antigen , concordance , sequence (biology) , computational biology , biology , genetics , computer science , antigen
Summary An HLA ‐ DQA 1 sequence‐based typing method reliant upon group‐specific amplification to achieve an unambiguous second‐field DQA 1 typing assignment is presented. Method validation, using 51 reference DNA samples covering 21 different DQA 1 alleles, showed 100% concordance with the reference types. This typing strategy has several important uses including identifying DQA 1 mismatches in kidney donor/recipient pairs to inform patient DQ antibody assignments.