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Pro‐ and anti‐inflammatory cytokine gene single‐nucleotide polymorphisms in inflammatory bowel disease
Author(s) -
LópezHernández R.,
Valdés M.,
Campillo J. A.,
MartínezGarcía P.,
Salama H.,
Bolarin J. M.,
Martínez H.,
MoyaQuiles M. R.,
Minguela A.,
SánchezTorres A.,
Botella C.,
Salgado G.,
Miras M.,
Carballo F.,
Muro M.
Publication year - 2015
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/iji.12160
Subject(s) - inflammatory bowel disease , allele , immunology , single nucleotide polymorphism , ulcerative colitis , cytokine , interleukin 10 , genotype , interleukin 18 , biology , medicine , disease , gene , genetics
Summary Anti‐inflammatory cytokines have an important role in disease, tumour and transplant processes. Alterations in the regulation of several cytokines have been implicated in a variety of inflammatory disorders, including IBD (inflammatory bowel disease) [ C rohn′s disease ( CD ) and ulcerative colitis ( UC )]. Cytokine polymorphisms are also known to affect the level of gene expression. Thus, the aim of this study was to determine the relationship between cytokine polymorphisms and the IBD pathologies in a S panish population. Polymorphisms analysis was performed using PCR ‐ SSOP using a microbeads luminex assay. The following polymorphisms were determined: TNF α [−238G/ A (rs361525) and −308 G / A (rs1800629)], IFN γ [+874 A / T (rs62559044)], TGF β [+869 C / T (rs1982073) and +915 G / C (rs1800471)], IL 10 [−1082 A / A (rs1800896), −592 A / C (rs1800872), −819 C / T (rs1800871)], IL 6 [−174 C / G (rs1800795)], IL 12p40 [3′ UTR −1188 A / C (rs3212227)], IL 1 α [−889 C / T (rs1800587)], IL 1 β [−511 C / T (rs1143634) and +3962 C / T (rs1143633)], IL 1 R [ P st‐1 1970 C / T ] and IL 1 RA [ M spa‐1 11100 C / T ]. No statistical differences in TNF α , IFN γ, TGF β, IL 10, IL 6, IL 1α, IL 1β, IL 1 R and IL 1 R a genotypes and allele distributions between the IBD groups and healthy controls were found. However, we observed significant differences in the 3′ UTR −1188 A / C polymorphism of IL 12p40 . So −1188 A allele was increased in patients with UC and the −1188 C allele (high IL 12p40 production) was increased in patients with CD with respect to controls. These data are in concordance with the fact that CD has been shown to be associated with a T h1 T ‐cell‐mediated inflammation model and high IL 12/ IFN γ production at histological affected sites. These data suggest that cytokine polymorphisms in TNF α , IFN γ, TGF β, IL 10, IL 6 and IL 1α, IL 1β, IL 1 R and IL 1 R a cytokine gene do not seem to be relevant in IBD susceptibility and IL 12p40 3′ UTR −1188 A / C polymorphism seems to be associated with a differential IBD development.