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The association of −330 interleukin‐2 gene polymorphism and HLA ‐ DR 15 allele in I ranian patients with multiple sclerosis
Author(s) -
Sayad A.
Publication year - 2014
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/iji.12132
Subject(s) - allele , genotyping , genotype , immunology , human leukocyte antigen , allele frequency , polymorphism (computer science) , medicine , genetics , biology , antigen , gene
Summary The purpose of this case–control study was to evaluate the frequencies and potential genetic susceptibility of the −330 IL 2 T and G alleles and HLA ‐ DRB 1*1501 allele in Iranian patients with multiple sclerosis ( MS ) compared to healthy controls. Two hundred and sixty Iranian patients with MS from medical genetics department of Sarem Women hospital were selected. Besides, 450 ethnically age‐ and sex‐matched healthy individuals without personal or family backgrounds of autoimmune disorders were enrolled as a control group. All polymorphisms were analysed using RFLP ‐ PCR technique. HLA ‐ DRB 1 genotyping was carried out by HISTO TYPE SSP high‐resolution Kits according to the manufacturer's suggestions. The frequency of the T allele at the −330 IL 2 polymorphism was significantly higher in patients with MS than controls ( OR : 2.45, 95 CI : 1.9–3, P = 4 × 10 −14 ). Moreover, the T/T genotype was more frequent in patients than in controls (51% vs. 30%). This study indicated that the −330 T IL 2 allele and the T/T genotype were related to increased plasma concentration of IL 2 and a higher risk of developing MS among Iranian patients. Carrying both the −330 T IL 2 and the HLA , DRB 1* 1501 alleles showed the most susceptibly effect to MS . Our data demonstrated −330 T IL 2 allele provided major susceptibility to MS and HLA ‐ DRB 1* 1501 allele had an additive effect. In addition, it seems that studies with larger sample size are required to bring about more authentic results. Our findings suggest that IL 2 gene polymorphisms influence the susceptibility to MS in I ranian patients.