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Role of TLR 4 C>1196T (Thr399Ile) and TLR 4 A>896G (Asp299Gly) polymorphisms in a North Indian population with asthma: a case‐control study
Author(s) -
Sinha S.,
Singh J.,
Jindal S. K.,
Birbian N.,
Singla N.
Publication year - 2014
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/iji.12115
Subject(s) - genotyping , genotype , asthma , immunology , allele , pathogenesis , immune system , biology , pathogen associated molecular pattern , receptor , population , lipopolysaccharide , genetics , polymorphism (computer science) , gene , innate immune system , medicine , pattern recognition receptor , environmental health
Summary Toll‐like receptor 4 ( TLR 4) is the most important TLR among the pattern recognition receptors which recognizes lipopolysaccharide of gram‐negative bacteria. They identify a highly conserved structure of microbes called pathogen‐associated molecular patterns and activate immune and inflammatory responses that have been shown to be involved in the pathogenesis of asthma. The role of TLR 4 gene polymorphisms in asthma was detected in a total of 964 individuals, including 483 healthy controls and 481 asthma patients from a North Indian population. The genotyping was carried out using polymerase chain reaction–restriction fragment length polymorphism method. Statistical analysis revealed that the heterozygous genotype as well as the mutant (T) allele of the TLR 4 C>1196T (Thr399Ile) polymorphism shows resistance towards asthma with OR = 0.70, 95% CI (0.49–0.99), P corrected value = 0.046 and OR = 0.72, 95% CI (0.52–0.98), P corrected value = 0.039, respectively. However, no association was found between the TLR 4 A>896G (Asp299Gly) polymorphism and asthma patients ( P > 0.05). This is the first study conducted in India conferring TLR 4 (Thr399Ile) polymorphism resistance towards asthma, while lack of association was found between TLR 4 (Asp299Gly) polymorphism and asthma in the studied North Indian population.