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Nucleotide sequence and polymorphism of MHC class II DQB exon 2 alleles in C hinese yakow ( B os grunniens × B os taurus )
Author(s) -
Xi D.,
Hao T.,
He Y.,
Leng J.,
Sun Y.,
Yang Y.,
Mao H.,
Deng W.
Publication year - 2014
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/iji.12109
Subject(s) - exon , biology , nonsynonymous substitution , genetics , allele , gene , microbiology and biotechnology , major histocompatibility complex , single nucleotide polymorphism , locus (genetics) , genotype , genome
Summary The objective of this study is to describe the gene characteristics of the bovine lymphocyte antigen ( B oLA)‐ DQB exon 2 locus in C hinese yakow ( B os grunniens × B os taurus ) and to compare it with previously reported patterns in other bovidae species to investigate genetic factors for disease resistance. The exon 2 of the MHC class II DQB gene was cloned and sequenced. It was revealed by sequence analyses that there are 36 DQB exon 2 alleles among 44 C hinese yakow. These alleles exhibited a high degree of nucleotide and amino acid polymorphism with most amino acid variations occurring at positions forming the peptide‐binding sites ( PBS ). The DQB loci were analysed for patterns of synonymous ( d S ) and nonsynonymous ( d N ) substitution. The Chinese yakow was observed to be under strong positive selection in the DQB exon 2 peptide‐binding sites ( d N = 0.147, P < 0.01). It appears that this variability among C hinese yakow confers the ability to mount immune responses to a wide variety of peptides or pathogens.