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HLA ‐A, ‐B and ‐ DRB 1 polymorphism in K oreans defined by sequence‐based typing of 4128 cord blood units
Author(s) -
Huh J. Y.,
Yi D. Y.,
Eo S.H.,
Cho H.,
Park M. H.,
Kang M. S.
Publication year - 2013
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/iji.12067
Subject(s) - haplotype , allele , typing , human leukocyte antigen , hla a , genetics , biology , locus (genetics) , genotype , allele frequency , hla drb1 , hla b , gene , antigen
Summary Human leucocyte antigen ( HLA ) alleles and haplotypes differ significantly among different ethnic groups, and high‐resolution typing methods allow for the detection of a wider spectrum of HLA variations. In this study, HLA ‐A, ‐B and ‐ DRB 1 genotypes were analysed in 4128 cord blood units obtained from Korean women using the sequence‐based typing method. A total of 44 HLA ‐A, 67 HLA ‐B and 48 HLA ‐ DRB 1 most probable alleles were identified. Of these, high‐frequency alleles found at a frequency of ≥5% were 6 HLA ‐A ( A*02:01 , A*02:06 , A*11:01 , A*24:02 , A*31:01 , A*33:03 ), 5 HLA ‐B ( B*15:01 , B*44:03 , B*51:01 , B*54:01 , B*58:01 ) and 7 HLA ‐ DRB 1 ( DRB 1*01:01 , DRB 1*04:05 , DRB 1*07:01 , DRB 1*08:03 , DRB 1*09:01 , DRB 1*13:02 , DRB 1*15:01 ) alleles. At each locus, A*02 , B*15 and DRB 1*04 generic groups were most diverse at allelic level, consisting of 8, 11 and 10 different alleles, respectively. Two‐ and three‐locus haplotypes estimated by the maximum likelihood method revealed 73 A‐B, 74 B‐ DRB 1 and 42 A‐B‐ DRB 1 haplotypes with frequencies of ≥0.3%. A total of 193 A‐B‐ DRB 1 haplotypes found at a frequency of ≥0.1% were presented, and the six most common haplotypes were A*33:03‐B*44:03‐ DRB 1*13:02 (4.6%), A*33:03‐B*58:01‐ DRB 1*13:02 (3.0%), A*24:02‐B*07:02‐ DRB 1*01:01 (2.7%), A*33:03‐B*44:03‐ DRB 1*07:01 (2.5%), A*30:01‐B*13:02‐ DRB 1*07:01 (2.2%) and A*24:02‐B*52:01‐ DRB 1*15:02 (2.1%). Compared with previous smaller scale studies, this study further delineated the allelic and haplotypic diversity in Koreans including low‐frequency alleles and haplotypes. Information obtained in this study will be useful for the search for unrelated bone marrow donors and for anthropologic and disease association studies.