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Relation of IL‐6, IL‐13 and IL‐15 gene polymorphisms to the rheumatoid factors, anti‐CCP and other measures of rheumatoid arthritis activity
Author(s) -
Pavkova Goldbergova M.,
Nemec P.,
Lipkova J.,
Jarkovsky J.,
Gatterova J.,
Ambrozkova D.,
Vasku A.,
Soucek M.,
Pavek N.
Publication year - 2014
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/iji.12065
Subject(s) - rheumatoid arthritis , gene , immunology , medicine , relation (database) , genetics , biology , data mining , computer science
Summary The aim of the study was to examine the relation between polymorphisms and serum levels of selected cytokines ( IL ‐6, IL ‐13 and IL ‐15), production of autoantibodies and factors describing rheumatoid arthritis ( RA ), such as DAS 28 and Total Sharp Score. A total of 156 patients with RA according to the ACR criteria, and 200 control subjects were recruited into the study. The measurements of CRP , anti‐ CCP , the presence of rheumatoid factors ( RF s), radiographs of both hands with calculation of Total Sharp Score ( TSS ) and DAS 28 were obtained from all patients with RA . In total, five polymorphisms in genes coding cytokines ( IL ‐6, IL ‐13 and IL ‐15) were detected. The levels of these selected cytokines were measured in serum using ELISA method. A significant difference in allele frequencies between patients with RA and controls was observed for IL ‐15 ‐267C/T polymorphism. A higher prevalence of heterozygote variants of IL ‐15 polymorphisms (14035A/T and ‐267C/T) in the RF IgG– and RF IgA–negative subgroups was observed. Furthermore, the association of polymorphisms in gene for IL ‐15 with circulating level of IL ‐15 (14035A/T and 367G/A) and with total RF and Ig‐specific RFs (‐267C/T) was found. The relation of IL ‐15 to RF s IgA, IgM, IgG and the measure of DAS 28 was proved. The frequency of the T allele of the IL ‐13 polymorphism ‐1112C/T was higher in subgroup with faster progression of the disease ( TSS /month ≥ 0.1). In conclusion, we present an association of IL ‐15 gene polymorphisms with the RFs including subtypes ( RF , IgG, IgA) underlined by the relation of increased IL ‐15 levels in circulation to RF s.