Premium
HLA ‐A, HLA ‐B, HLA ‐ DRB 1 allele and haplotype frequencies in 6384 umbilical cord blood units and transplantation matching and engraftment statistics in the Zhejiang cord blood bank of China
Author(s) -
Wang F.,
He J.,
Chen S.,
Qin F.,
Dai B.,
Zhang W.,
Zhu FM.,
Lv HJ.
Publication year - 2014
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/iji.12064
Subject(s) - haplotype , umbilical cord , immunology , human leukocyte antigen , allele , cord blood , allele frequency , transplantation , hla a , linkage disequilibrium , biology , genetics , medicine , antigen , gene
Summary Umbilical cord blood ( UCB ) is a widely accepted source of progenitor cells, and now, many cord blood banks were established. Here, we analysed the HLA ‐A, HLA ‐B and HLA ‐ DRB 1 allele and haplotype frequencies, HLA matching possibilities for searching potential donors and outcome of UCB transplantations in Zhejiang cord blood bank of China. A total of 6384 UCB units were characterized for 17 HLA ‐A, 30 HLA ‐B and 13 HLA ‐ DRB 1 alleles at the first field resolution level. Additionally, B*14, B*15 and B*40 were typed to the second field level. A total of 1372 distinct A‐B‐ DRB 1 haplotypes were identified. The frequencies of 7 haplotypes were more than 1%, and 439 haplotypes were <0.01%. A*02‐B*46‐ DRB 1*09, A*33‐B*58‐ DRB 1*03 and A*30‐B*13‐ DRB 1*07 were the most common haplotypes, with frequencies of 4.4%, 3.3%, and 2.9%, respectively. Linkage disequilibrium( LD ) analysis showed that there were 83 A‐B, 106 B‐ DRB 1, 54 A‐ DRB 1 haplotypes with positive LD , in which 51 A‐B, 60 B‐ DRB 1, 32 A‐ DRB 1 haplotypes exhibited a significant LD ( P < 0.05). In 682 search requests, 12.9%, 40.0% and 42.7% of patients were found to have 6 of 6, 5 of 6 and 4 of 6 HLA ‐A, HLA ‐B and HLA ‐ DRB 1 matching donors, respectively. A total of 30 UCB units were transplanted to 24 patients (3 patients not evaluated due to early death); 14 of 21 patients (66.7%) engrafted. This study reveals the HLA distribution and its transplantation application in the cord blood bank of Zhejiang province. These data can help to select potential UCB donors for transplantation and used to assess the scale of new cord blood banking endeavours.