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Development of protease nanobiocatalysts and their application in hydrolysis of sunflower meal protein isolate
Author(s) -
Katić Katarina,
Banjanac Katarina,
Simović Milica,
Ćorović Marija,
Milivojević Ana,
Marinković Aleksandar,
Bezbradica Dejan
Publication year - 2021
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/ijfs.15189
Subject(s) - hydrolysis , chemistry , protease , chromatography , sunflower , cyanuric chloride , enzyme , biochemistry , organic chemistry , biology , agronomy
Summary In this study, the suitability of fumed silica nanoparticles (FNS) and its derivatives (amino‐modified FNS (AFNS), cyanuric chloride‐activated AFNS (CCAFNS) and epoxy‐modified FNS (GFNS)), for covalent immobilisation of two commercial protease preparations Alcalase ® and Flavourzyme ® was investigated. The highest hydrolytic activities of immobilised preparations were 25 IU g −1 support (Alcalase‐GFNS) and 2.95 IU g −1 support (Flavourzyme‐CCAFNS). Furthermore, the immobilised preparations showed 43% and 20% of initial specific activities of commercial protease preparations, respectively. Flavourzyme‐CCAFNS also exhibited the highest exopeptidase activity of 22.83 L‐ p NAU g −1 support. Finally, these two nanobiocatalysts were successfully applied for hydrolysis of sunflower meal protein isolate (SMPI), providing two times higher hydrolysis yields in comparison to free enzymes, justifying the applied immobilisation process. Namely, the highest hydrolysis yield (30%) was gained by the sequential hydrolysis with Alcalase‐GFNS and Flavourzyme‐CCAFNS, which resulted in the formation of small hydrophobic and hydrophilic peptides, ≤5 kDa, confirmed by HPLC analysis and electrophoretic separation.