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Determination of aflatoxins, deoxynivalenol, ochratoxin A and zearalenone in organic wheat flour under different storage conditions
Author(s) -
Annunziata Loredana,
Schirone Maria,
Visciano Pierina,
Campana Guido,
De Massis Maria Rosaria,
Migliorati Giacomo
Publication year - 2021
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/ijfs.15042
Subject(s) - zearalenone , ochratoxin a , mycotoxin , aflatoxin , chemistry , detection limit , chromatography , extraction (chemistry) , european commission , food science , european union , business , economic policy
Summary Samples of flour derived from an organic wheat seed cultivated in the region Abruzzo (Central Italy) were examined for the detection of aflatoxins, deoxynivalenol, ochratoxin A and zearalenone after storage in the presence of oxygen or under vacuum, for one year. They were analysed by high‐performance liquid chromatography with fluorescence detection for aflatoxins, ochratoxin A and zearalenone and with diode array detector for deoxynivalenol. An immunoaffinity column clean‐up was applied for all the compounds, except for aflatoxin determination, which required a solid‐phase extraction. Recoveries (range: 76–107%) and within‐laboratory reproducibility (RSD% < 10.3) met the performance criteria of Commission Regulation (EU) No. 519/2014 amending Commission Regulation (EC) No. 401/2006 for the detection of mycotoxins in foodstuffs. Analytes were below the detection limit of the method, and this result was probably due to the quality of organic seed and good agricultural practices performed for the prevention of mycotoxin formation.