Premium
One‐step triplex high‐resolution melting (HRM) analysis for rapid identification of Atlantic cod ( Gadus morhua ), Alaska pollock ( Gadus chalcogrammus ) and haddock ( Melanogrammus aeglefinus )
Author(s) -
Chen Xiaoye,
Fu Mingyang,
Xu Wenjie,
Huang Manhong,
Cui Xiaowen,
Cao Min,
Xiong Xiaohui,
Xiong Xiong
Publication year - 2021
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/ijfs.14924
Subject(s) - gadus , haddock , atlantic cod , biology , fishery , gadidae , zoology , fish <actinopterygii>
Specific primers were designed for Gadus morhua , Gadus chalcogrammus and Melanogrammus aeglefinus . Specificity of these specific primers was confirmed. Triplex HRM assay allowed easy and quick differentiation of the three species. G. morhua was detected in 30.8% (4/13) commercial codfish products. G. chalcogrammus was detected in 38.5% (5/13) commercial codfish products.