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Deciphering the role of selenium‐enriched rice protein hydrolysates in the regulation of Pb 2+ ‐induced cytotoxicity: an in vitro Caco‐2 cell model study
Author(s) -
Zhu Yiqing,
Ding Jian,
Shi Yi,
Fang Yong,
Li Peng,
Fan Fengjiao,
Wu Jian,
Hu Qiuhui
Publication year - 2021
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/ijfs.14657
Subject(s) - selenium , cytotoxicity , caco 2 , hydrolysate , viability assay , chemistry , absorption (acoustics) , in vitro , toxicity , chelation , nuclear chemistry , biochemistry , materials science , organic chemistry , hydrolysis , composite material
Summary Lead (Pb), a well‐known toxic metal, has a potential of adverse effects on human health. Selenium (Se) can reduce the toxicity caused by heavy metals. In this study, we investigated the protection mechanism of the purified Se‐enriched rice protein hydrolysates (SPHs‐2) on the Pb 2+ ‐induced cytotoxicity. A Caco‐2 cell model was established to characterise the effect of SPHs‐2 on Pb 2+ absorption in the simulated small intestine. Results showed that 1000 μg mL −1 of SPHs‐2 treatment could significantly increase cell viability by 19.43% in Pb 2+ ‐treated Caco‐2 cells ( P < 0.05). Furthermore, the absorption of Pb 2+ was successfully retarded by incorporating SPHs‐2. Interestingly, selenomethionine (SeMet) was identified as a major Se species in SPHs‐2 by HPLC‐ICP‐MS, and SeMet played a crucial role in regulating Pb 2+ intestinal absorption via forming complexes. In light of this, SPHs‐2 may serve as a potential Pb 2+ ‐chelating peptide for developing novel functional food and protecting human health.