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Rapid detection and toxicity assessment of ochratoxin A by Photobacterium leiognathi in drinking water
Author(s) -
Li Liang,
Wu Shizheng,
Yang Panpan,
Liu Qianyu,
Tang Shuze
Publication year - 2020
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/ijfs.14411
Subject(s) - chemistry , photobacterium phosphoreum , ochratoxin a , microbiology and biotechnology , gel electrophoresis , chromatography , toxicity , mycotoxin , biochemistry , food science , biology , organic chemistry
Summary This study was aimed to develop a rapid technique for detection of ochratoxin A ( OTA ) by Photobacterium leiognathi ( P. leiognathi ) based on its inhibition of luminescence on P. leiognathi . The freeze‐dried powder of P. leiognathi was incubated and grown aerobically in sterile liquid medium at 28 °C for 20 h. Optical cell density ( OD ) at wavelength of 610 nm was measured using UV spectrometer every 2 h. Different concentrations of standard OTA solution were used to measure its luminescence inhibition and calculate its half maximal inhibitory concentration ( IC 50 ). Scanning electron microscopy ( SEM ), flow cytometry ( FCM ), sodium dodecyl sulphonate polyacrylamide gel electrophoresis ( SDS ‐ PAGE ), DNA extraction and gel electrophoresis were used to observe the performance of P. leiognathi under the treatment of OTA . A correlation ( R 2 > 0.98) was obtained between the relative luminosity unit of P. leiognathi and OTA concentration in the range of 0.01–20 mg L −1 with recoveries of 80.8–87.4%. The effects of OTA on P. leiognathi are time‐dependent, and the IC 50 value of 12.71 mg L −1 at 30 min demonstrated its good sensitivity to OTA . The cells of P. leiognathi under 40 mg L −1 OTA exposure for 30 mins showed morphological alterations, protein damage, apoptosis and necrosis. The aforementioned results indicate that biological assay is a promising and alternative method used for rapidly monitoring the sudden pollution of OTA in the early emergency warning of drinking water system.